Figure 5.

Immunofluorescence staining of KLF8 in H/R-exposed HTR-8/SVneo cells. HTR-8/SVneo cells were analyzed by anti-KLF8 staining of the KLF8 protein (green) and propidium iodide staining of the nuclei (red), followed by fluorescence microscopy. Control, nontreated cells were maintained under normal conditions for 48 hours. Almost all the cells showed exclusive nuclear localization. The H/R cells were cultured under hypoxic conditions (2% oxygen) for 8 hours, followed by reoxygenation for 16 hours, and the H/R intervention described above was repeated twice. A larger proportion of cells showed both nuclear and cytoplasmic localization. Scale bar, 75 μm. H/R, hypoxia–reoxygenation; KLF8, Krüppel-like factor 8.