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. 2014 Jan;21(1):82–88. doi: 10.1177/1933719113488449

Figure 3.

Figure 3.

A, Representative images of immunoblotting against β-actin, CD44s, and CD10 expressed by ESCs, eESCs, and CSCs. The expression of CD10 was suppressed by the siRNA. B-D, Adhesion assays using ESCs (B), eESCs (C) and CSCs (D) to hyaluronan. Each ESC from patients 1 to 5 (pt 1-5), and each eESC and CSC from the patients with endometriosis (pt e1-e5) showed decreased adhesion following treatment with progesterone, 17β-estradiol, and dibutyryl cAMP (EP), which was partially recovered by the suppression of CD10 expression using siRNA (EP+si). A neutralizing antibody targeting CD44 attenuated the adhesion of cells (EP+si+Ab). E, A comparison of the adhesion rates in ESCs, eESCs, and CSCs. ESCs indicates the stromal cells taken from the eutopic endometrium of patients without endometriosis; eESCs, the stromal cells taken from the eutopic endometrium of patients with endometriosis ; CSCs, the stromal cells taken from the chocolate cyst linings of the ovaries of patients with endometriosis ; cAMP, cyclic adenosine monophosphate; siRNA, small interfering RNA.