Figure 5.

Expression of pituitary adenylate cyclase-activating polypeptide (PACAP; A), PACAP receptor (PAC1; B), GnRHR (C), and progesterone receptor (PRB; D) in rat pituitary cells treated with vehicle, 10, or 100 nmol/L E₂ for 6 or 24 hours, and 100 nmol/L gonadotropin-releasing hormone (GnRH) analog (or equal volume of H₂O as control) was included for the last 6 hours of incubation. The samples were measured as described in Figure 1. Bars represent the mean ± standard error of the mean of each group (n ≥ 3). Bars at the same time point with different letters vary significantly (P < .05).