Table 1.
Genes | Assay ID/Primer Sequences |
---|---|
Tagman assays | |
AR | Rn00560747_m1 |
FSHβ | Rn01484594_m1 |
GnRHR | Rn00578981_m1 |
PACAP | Rn00566438_m1 |
PAC1 | Rn00591653_m1 |
SYBR green method | |
PRB | Forward: 5′-CCAATACCGATCTCCCTGGAC-3′ |
Reverse: 5′-CTTCCACTCCAGAGAAAGCTCC-3′ |
Abbreviations: AR, androgen receptor; GnRHR, GnRH receptor; FSHβ, follicle-stimulating hormone β; PACAP, pituitary adenylate cyclase-activating polypeptide; PAC1, PACAP receptor; PCR, polymerase chain reaction; PRB, progesterone receptor.