. 2013 Dec 5;8(12):e80545. doi: 10.1371/journal.pone.0080545

Table 1. Number of OVA-specific CD4⁺ T cells and total lymphocytes.

Route of immunization	Lymphoid organ^a	Time	OVA-specific T cells^b	Total Lymphocytes
Vaginal	ILN	0	15.7±6.2	2303±925
		57	74.5±34.5	4385±1300
		72	106±64^*** ^c	5017±2600
	CLN	0	31.9±21	4264±1322
		57	8.8±3.6 ^*	3890±1190
		72	16±9.1	4888±2400
	SPL	0	185.5±24.5	40748±11482
		57	52.2±19 ^***	30052±5117
		72	157±70	47902±35887
Nasal	CLN	0	31.9±21	4264±1322
		57	26±28	4832±1219
		72	79.5±47.6 ^*	7636±3777
	ILN	0	15.7±6.2	2303±925
		57	1.7±0.7 ^***	1909±541
		72	4.5±3.2 ^***	1735±1059
	SPL	0	185.5±24.5	40748±11482
		57	68.2±16.4 ^*	47167±10180
		72	186.4±118	43000±15916

ILN: Iliac lymph nodes (subiliac, medial and external), CLN: cervical lymph nodes (superficial and posterior) and SPL: spleen.

Absolute number of OVA-specific CD4⁺ T cells calculated as [(percentage of CD4⁺CFSE⁺×absolute number of lymphocytes)/100] for each lymphoid organ. OVA-specific T cells and total lymphocytes absolute numbers are to be multiplied×10∧3.

One-way analysis of variance (ANOVA) and Tukey's post test were used to compare the number of OVA-specific CD4⁺ T cells present in lymphoid organs at different time points.

P<0.05;

^***

P<0.001.

Table 1. Number of OVA-specific CD4+ T cells and total lymphocytes.

Table 1. Number of OVA-specific CD4⁺ T cells and total lymphocytes.