Figure 3. Phylogenetic tree based on 16S rRNA gene sequences (1376 nts) showing the evolutionary relationships among the obtained “B. hampsonii” isolates and Brachyspira spp. reference strains.

The tree was constructed by means of the neighbour-joining method. Red circles indicate the two clusters containing the “B. hampsonii” reference isolates. The blue circle indicates the cluster comprising the B. innocens and B. murdochii reference strains used.