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. 2013 Dec 9;8(12):e81329. doi: 10.1371/journal.pone.0081329

Table 7. List of Promoter Single Nucleotide Polymorphisms Screened.

Name SNP ID Primers Annealing Temperature (°C) Product Enzyme Restriction Fragment
−1447A>G rs4508917 5′-TTGGTCAGGGAATGGAAAAG-3′ 55 290 bp SacI 145/145 bp
5′-CGGTTTCCCACAGCTAATTC-3′
−135G>A rs56061981 5′-CCGTTCATGTTTTGGAAAGTGA-3′ 48 123 bp BstBI 61/62 bp
5′-GGGAAGTCCCATGTTGCAGATT-3′
−872G>A rs4256246 5′-TGAAATTAAGTTTTGCCACGA-3′ 52 155 bp NcoI 22/133 bp
5′-GGAAACAGGTTGATTTACCATG-3′
−1596C>T rs74810361 5′-GCAGATACTGTCTCAGAACCTGGTA-3′ 57 499 bp XbaI 174/325 bp
5′-TGTCACCATCTCTCATTTTGATTGT-3′

SNP  =  single nucleotide polymorphism; ID  =  identification; bp  =  base pairs