Table 7. List of Promoter Single Nucleotide Polymorphisms Screened.
Name | SNP ID | Primers | Annealing Temperature (°C) | Product | Enzyme | Restriction Fragment |
−1447A>G | rs4508917 | 5′-TTGGTCAGGGAATGGAAAAG-3′ | 55 | 290 bp | SacI | 145/145 bp |
5′-CGGTTTCCCACAGCTAATTC-3′ | ||||||
−135G>A | rs56061981 | 5′-CCGTTCATGTTTTGGAAAGTGA-3′ | 48 | 123 bp | BstBI | 61/62 bp |
5′-GGGAAGTCCCATGTTGCAGATT-3′ | ||||||
−872G>A | rs4256246 | 5′-TGAAATTAAGTTTTGCCACGA-3′ | 52 | 155 bp | NcoI | 22/133 bp |
5′-GGAAACAGGTTGATTTACCATG-3′ | ||||||
−1596C>T | rs74810361 | 5′-GCAGATACTGTCTCAGAACCTGGTA-3′ | 57 | 499 bp | XbaI | 174/325 bp |
5′-TGTCACCATCTCTCATTTTGATTGT-3′ |
SNP = single nucleotide polymorphism; ID = identification; bp = base pairs