Figure 1. FV-induced splenomegaly in mice immunized with adenoviral vectors.

CB6F1 mice were immunized twice with Ad.pIXgp70 in combination with adenoviral vectors encoding specific interleukins. Mice were immunized first with Ad5 vectors and after 4 weeks, mice were boost-immunized with Ad5F35 vectors. Mice of the group Ad.pIXgp70 received in addition a GFP-encoding or empty adenoviral vector so that the total amount of adenoviral particles in all vaccinated groups was the same. Three weeks after the second immunization, mice were challenged with a high dose of FV and spleens were removed and weighed 3 weeks after challenge infection. Statistically significant differences (P < 0.05; Kruskal-Wallis one-way analysis of variance on ranks with Dunns multiple comparison procedure) compared to unvaccinated control mice (#) are indicated. Each dot shows an individual mouse, the horizontal lines indicate mean values. Data are results of two independent experiments with similar outcome.