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Time-course of the acute antinociceptive effect of graded doses of EM2 (a) and ACHC-EM2 (b) in rat tail-flick assay. The peptides were administered icv and assayed as described in Methods. Control groups received CSF. Data are shown as mean ± S.E.M; n ≥ 6. Statistical significance was determined by ANOVA and set at *P < 0.05 compared to appropriate control values.
