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. 2013 Aug 13;23(1):24–39. doi: 10.1093/hmg/ddt393

Figure 4.

Figure 4.

Increased angiogenesis-related gene expression and enhanced capillary abundance in ISCU myopathy patient muscles. qRT-PCR revealed significant increases in (A) VEGFB (average 2.7-fold), (B) the VEGF receptor KDR (average 4-fold), (C) hypoxia-inducible factor 2α (EPAS1/HIF-2α; average 3.3-fold) and (D) fibroblast growth factor 2 (FGF2; average 9-fold) in ISCU myopathy patient biopsies. The non-transformed 2−ΔΔCt values were analyzed by one-way ANOVA with Holm–Sidak post hoc test. *P < 0.05; **P < 0.01. (E) Immunoblot analysis of muscle biopsy protein extracts demonstrated increased VEGF protein expression in ISCU myopathy patients compared with control. Immunohistochemistry showed increased abundance of brown-colored CD31-positive capillaries perfusing myofibers in muscle sections taken from an ISCU myopathy patient (F), compared with the healthy heterozygous offspring of this individual (G).