Figure 2.

Acetylation of KXGS motifs regulates tau assembly. (A) WT or K259/290/321/353R (4KR) mutant proteins were acetylated and incubated with dextran sulfate to promote polymerization, which was detected by thioflavin S. (B) WT or K259/290/321/353Q (4KQ) mutant proteins were incubated with dextran sulfate, and tau assembly was detected with thioflavin S. (C) WT or 4KQ proteins were acetylated and incubated with HDAC6 where indicated. Dextran sulfate was used to promote tau polymerization, which was detected by thioflavin S. All data are presented as mean ± SEM. *P < 0.001.