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. 2013 Aug 19;23(1):104–116. doi: 10.1093/hmg/ddt402

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© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — KIGS motifs are hypoacetylated and hyperphosphorylated in AD. (A) Levels of ac-KIGS, 12E8 and total human tau (E1) were assessed in frontal cortex from patients with AD compared to control cases. Recombinant tau (rTau) was acetylated in vitro and utilized as a positive control for the ac-KIGS antibody. (B and C) ac-KIGS levels were significantly decreased in AD when normalized to total tau (B; t = 3.1, P = 0.01), as well as when normalized to tubulin (C; t = 2.9, P = 0.02). (D) MSD sandwich immunoassays were performed to measure ac-KIGS in brain homogenates. Results from the ac-KIGS immunoassay were normalized to results from the total tau assay, which again revealed a decrease in ac-KIGS tau in AD (t = 5.3, P = 0.0003). (E) 12E8 levels were also measured by MSD immunoassay, and the ratio of ac-KIGS to 12E8 is significantly decreased in AD (t = 7.5, P < 0.0001). All data are presented as mean ± SEM. *P < 0.05, **P < 0.001, ***P < 0.0001.