Effect of blockade of VMH B2AR receptors on the B2AR agonist response. GIR (a) and plasma concentrations of the counter-regulatory hormones adrenaline (b) and glucagon (c) in rats receiving microinjection of the artificial ECF vehicle (control; n=8), or the B2AR antagonist ICI-118,551 (n=8), during the hyperinsulinaemic–hypoglycaemic glucose clamp. The arrow indicates time when B2AR agonist was delivered. Circles and grey bars, B2AR agonist; squares and white bars, control. Results are presented as means ± SEM. *p<0.05 and ***p<0.001 vs control