Table 1.
Summary of results from histological diagnosis, HPV-DNA and mRNA tests, HPV viral load, CISH, and p16/Ki67 dual stain.
| Case | Histological diagnosis | HR HPV-DNA test result |
HC2 viral load (RLU/CO)* |
E6/E7 mRNA test result | Type specific mRNA test result** | HPV CISH† signal | P16/Ki67 Dual stain result |
|---|---|---|---|---|---|---|---|
| 1 | CIN1 | Positive | 63.5 | Negative | — | P | Negative |
| 2 | CIN1 | Positive | 516.61 | Negative | — | D | Negative |
| 3 | CIN3 | Positive | 15.79 | Negative | — | Negative | Positive |
| 4 | CIN2 | Positive | 146.77 | Positive | 16 | D/P | Positive |
| 5 | CIN2 | Positive | 1283.97 | Negative | — | D/P | Positive |
| 6 | CIN1 | Positive | 298.55 | Negative | — | D | Negative |
| 7 | CIN3 | Positive | 1529.35 | Positive | 16, 31 | D/P | Positive |
| 8 | CIN3 | Positive | 2207.8 | Positive | 31 | D/P | Positive |
| 9 | CIN1 | Positive | 2.06 | Negative | — | D | Negative |
| 10 | CIN2 | Positive | 216.93 | Positive | 31 | D/P | Positive |
| 11 | CIN1 | Positive | 6.7 | Negative | — | Negative | Negative |
| 12 | CIN2 | Positive | 84.32 | Positive | 31 | Negative | Positive |
| 13 | CIN3 | Positive | 10.56 | Positive | 16 | D/P | Positive |
| 14 | CIN3 | Positive | 338.02 | Positive | 16 | D/P | Positive |
| 15 | CIN1 | Positive | 1211.84 | Positive | 33 | D/P | Positive |
| 16 | CIN3 | Positive | 3.3 | Positive | 16 | P | Positive |
| 17 | CIN1 | Positive | 155.79 | Positive | 16 | D | Negative |
| 18 | CIN1 | Positive | 28.81 | Negative | — | Negative | Positive |
| 19 | CIN2 | Positive | 34.82 | Negative | — | D | Positive |
| 20 | CIN3 | Positive | 913.36 | Negative | — | D | Positive |
| 21 | CIN2 | Positive | 1536.02 | Positive | 16 | P | Negative |
| 22 | CIN2 | Positive | 107.22 | Positive | 18, 45 | D/P | Positive |
| 23 | CIN1 | Positive | 75.86 | Negative | — | D/P | Negative |
| 24 | CIN2 | Positive | 675.75 | Positive | 16 | D/P | Positive |
| 25 | CIN2 | Positive | 596.84 | Positive | 16 | D/P | Positive |
| 26 | CIN3 | Positive | 1914.17 | Positive | 16 | D/P | Positive |
| 27 | CIN2 | Positive | 570.26 | Positive | 16, 45 | D/P | Positive |
| 28 | CIN1 | Positive | 783.56 | Negative | — | D | Negative |
| 29 | CIN1 | Negative | — | Negative | — | Negative | Negative |
| 30 | CIN3 | Positive | 3.93 | Positive | 16 | P | Positive |
| 31 | CIN3 | Positive | 968.56 | Positive | 31 | D/P | Positive |
| 32 | CIN1 | Positive | 926.05 | Positive | 31 | Invalid | Negative |
| 33 | CIN2 | Negative | — | Positive | 31 | Invalid | Positive |
| 34 | CIN1 | Positive | 45 | Negative | — | D | Negative |
| 35 | CIN3 | Positive | 117 | Positive | 16 | D/P | Positive |
| 36 | CIN1 | Positive | 237.48 | Positive | 16 | Invalid | Negative |
| 37 | CIN1 | Positive | 5.31 | Positive | 18, 31 | Negative | Negative |
| 38 | CIN1 | Positive | 204.42 | Positive | 45 | Negative | Negative |
| 39 | CIN3 | Positive | 663.26 | Positive | 33 | Negative | Positive |
| 40 | CIN3 | Positive | 38.61 | Positive | 16 | Negative | Positive |
| 41 | CIN1 | Positive | 10.11 | Positive | 33 | D/P | Positive |
| 42 | CIN1 | Positive | 137.88 | Negative | — | D | Negative |
| 43 | CIN3 | Positive | 6.21 | Positive | 16 | Negative | Positive |
| 44 | CIN2 | Positive | 2663.26 | Positive | 31 | D/P | Positive |
| 45 | SCC | Positive | 1549.74 | Positive | 33 | D/P | Positive |
| 46 | CIN2 | Positive | 894.17 | Positive | 16 | P | Positive |
| 47 | CIN1 | Positive | 20.47 | Negative | — | Negative | Negative |
| 48 | CIN1 | Positive | 26.23 | Negative | — | Negative | Negative |
| 49 | CIN1 | Positive | 787.16 | Positive | 31 | Negative | Negative |
| 50 | CIN1 | Positive | 676.46 | Positive | 16 | D/P | Negative |
| 51 | CIN3 | Positive | 2.36 | Positive | 16 | P | Positive |
| 52 | CIN3 | Positive | 1.45 | Positive | 18 | P | Positive |
| 53 | CIN3 | Positive | 111.95 | Positive | 16, 18 | D/P | Positive |
| 54 | CIN3 | Positive | 544.41 | Positive | 16 | D/P | Positive |
| 55 | CIN2 | Positive | 663.21 | Positive | 16 | D/P | Positive |
| 56 | CIN1 | Negative | — | Negative | — | Negative | Negative |
| 57 | CIN2 | Positive | 1569.56 | Positive | 18 | D/P | Positive |
| 58 | CIN3 | Positive | 758.66 | Positive | 16 | D/P | Positive |
| 59 | CIN2 | Positive | 130.13 | Positive | 16 | D/P | Positive |
| 60 | CIN3 | Positive | 87.01 | Positive | 16 | P | Positive |
| 61 | CIN3 | Positive | 968.56 | Positive | 31 | D/P | Positive |
| 62 | CIN2 | Positive | 6.21 | Positive | 16 | P | Positive |
| 63 | CIN3 | Positive | 24.01 | Positive | 16 | P | Positive |
*Relative light unit in relation to control (RLU/CO).
**HPV genotype(s) detected by Nuclisens EasyQ HPV mRNA test.
†D: diffuse; P: punctated; D/P: mixed diffuse/punctated.