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. Author manuscript; available in PMC: 2013 Dec 10.
Published in final edited form as: Exp Cell Res. 2012 Jun 8;318(16):10.1016/j.yexcr.2012.06.001. doi: 10.1016/j.yexcr.2012.06.001

Fig. 3.

Fig. 3

EGFR tyrosine phosphorylation is regulated by reactive oxygen. (A) MDA-MB-468 cells were treated with 0.5 µM gefitinib for 30min and/or 50mM N-acetyl cysteine for the last 10 min as indicated. Cell lysates were immunoprecipitated using anti-EGFR antibodies and immunoblotted with anti-EGFR or anti-phosphotyrosine antibodies. (B) SUM149 cells were treated with 0.5 µM gefitinib and/or 5mM H2O2. Cell lysates were immunoprecipitated using anti-EGFR antibodies and immunoblotted with anti-EGFR or anti-phosphotyrosine antibodies. Control=vehicle control treated; gef=gefitinib treated; NAC=N-acetyl cysteine treated; IB=immunoblot; and IP=immunoprecipitation.