Table 2.
Orthogonal planes and tilted planes recorded by ADAPT-NMR on Bruker DRX 500 MHz spectrometer.
| (A) Chlorella ubiquitin
| |||
|---|---|---|---|
| Experiment | Orthogonal planes | Angles of tilted planes | Time |
| 1H,15N-HSQC | 90° | - | 40 min |
| HNCO | 0° | 73°, 81°, | 53 min |
| HN(CO)CA | 0° | 76°, 50°, (30°)1 | 2 h 30 min |
| HNCA | 0° | 35°, 58°, 122°, 45°, (17°)1 | 1 h 56 min |
| CBCA(CO)NH | 0° | 46°, 39°, 32°, (54°, 28°, 62)1 | 3 h 38 min |
| HN(CA)CB | 0° | 37°, 50°, 71°, 22° | 3 h 16 min |
| HN(CA)CO | 0° | 49°, 72°, 37° | 5 h 7 min |
| ADAPT-NMR running between data collections | 2 h (approx) | ||
|
| |||
| Total time | 20 h (approx) | ||
| (B) BRPF1 bromodomain
| |||
|---|---|---|---|
| Experiment | Orthogonal planes | Angles of tilted planes | Time (h) |
| 1H,15N-HSQC | 90° | - | 39 min |
| HNCO | 0° | 16°, 54°, | 51 min |
| HN(CO)CA | 0° | 29°, 20°, 56° | 1 h 20 min |
| HNCA | 0° | 24°, 52°, 42°, 34° | 3 h 9 min |
| CBCA(CO)NH | 0° | 29°, 70°, 47°, 40°, (18°, 53°, 43°, 34)1 | 5 h 32 min |
| HN(CA)CB | 0° | 18°, 63°, 54°, 43°, 30°, (23°, 70)1 | 26 h 52 min |
| HN(CA)CO | 0° | 16°, 58°, 48°, 32°, 38°, (40°, 66°, 20) 1 | 12 h 11 min |
| ADAPT-NMR running between data collections | 4 h (approx) | ||
|
| |||
| Total time | 55 h (approx) | ||
1
Tilt angles in parentheses were recorded after the first continuous recording of tilted planes in the experiment list queue. The ADAPT-NMR engine specified these experiment types and angles on-the-fly to the ADAPT_NMR_run AU program.