FIG. 1.

Experimental design for study 1 exposure to TCDD at GD15, 4 weeks, and 9 weeks of age with postovariectomy hormone treatment. C57BL6/J mice were time mated/copulation plug positive (GD 0.5). At GD15, pregnant dams were orally administered vehicle (corn oil) or 10 µg/kg of TCDD in corn oil. Animals were weaned at 3 weeks of age. Offspring were orally administered vehicle or TCDD (10 µg/kg) at 4 weeks and 9 weeks of age. Mice were ovariectomized at 12 weeks of age, and a placebo or E2 pellet (0.5mg, 21-day release/mouse) was implanted. Days 6–10 after the pellet, animals received corn oil or P4 (1mg/mouse) subcutaneously for 4 days prior to sacrifice 11 days later.