Skip to main content
. 2013 Sep 30;136(2):467–477. doi: 10.1093/toxsci/kft214

FIG. 2.

FIG. 2.

Cytotoxicity and cell cycle changes in response to Co(II). A, Caspase 3/7 activity in H460 cells treated with Co(II) and Ni(II) for 24h (means ± SD for 4 independent determinations). In samples labeled Co + EDTA and Ni + EDTA, assay reagents were added after 5-min chelation of metals with 2mM EDTA. B, Formation of active (cleaved) caspase-7 in H460 cells treated with Ni(II) and Co(II) for 24h. C, Changes in the number of attached cells at the end of 24-h exposures relative to the pre-exposure level (“24 h” line) and after 24-h recovery relative to 0-h recovery (“24 + 24 h” line). The amounts of cells were determined by the CyQUANT reagent. Data are means ± SD for 4 independent determinations. In most cases, error bars were smaller than data symbols. D, Leakage of cellular LDH during 24-h exposure (“24 hr” line) and during 24-h recovery (“24 + 24 hr” line). Data and means ± SD for 4 independent determinations. E, Activity of LDH standard preincubated with or without 400μM Co(II) for 2h (means ± SD for 4 replicates). F, Westerns blots of protein extracts from H460 cells treated with Ni and Co for 24h. Bleo: cells were treated with 30 μg/ml bleomycin for 24h. G, Representative 2-parameter fluorescence-activated cell sorting (FACS) profiles of H460 cells treated with 0–400μM Co(II) for 24h. EdU incorporation is a measure of DNA replication, whereas propidium iodide is a general DNA stain. EdU (10μM) was added for 30min after removal of Co(II)-containing media. S-phase values are means ± SD for 3 independent samples. H, FACS profiles of EdU incorporation into H460 cells treated with 0–400μM Co(II) for 24h.