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. 2013 Sep 30;136(2):467–477. doi: 10.1093/toxsci/kft214

FIG. 3.

FIG. 3.

Comparison of Co(II) and Ni(II) cytotoxicity in H460 cells. In all experiments, cells were treated with metals for 24h in complete growth media. Viability was assessed by the determination of the metabolic activity of cell populations using the CellTiter-Glo luminescent assay, which measured total cellular ATP levels in a sample. Changes in cell viability at different times after exposure to Ni(II) (A) and Co(II) (B). Data are means ± SD for 6 independent determinations. C, Cell viability at 72h postexposure (means ± SD for 6 independent determinations). D, Clonogenic survival of H460 cells (means ± SD for 3 independent determinations). Clonogenic viability assesses the ability of cells to retain long-term proliferative activity, as scored by the presence of colonies resulting from proliferation of individual cells. Cells were treated with metals in mass cultures for 24 h, 400 cells from combined populations of attached and floating cells were seeded per 60-mm dish and then grown for 7 days to form visible colonies.