FIG. 5.

qRT-PCR for apoptosis-related genes in Co(II)-treated H460 cells. Total RNA was isolated from cells expressing nonspecific (sh-SCR) and p53-targeting shRNA (sh-p53) following Co(II) treatments for 24h. Data are means ± SD for 2 independent RNA preparations (**p < 0.01 relative to the same concentration in sh-SCR samples). A, mRNA levels for proteins associated with caspase-9 activation. B, Gene expression for antiapoptotic MCL1 and BCL2. C, Gene expression for proapoptotic BAX- and BH3-domain-containing activators.