FIGURE 4.

Unbinding rate measured in normal lung tissue and in emphysematous lung tissue

Unbinding rate (K_off) measured in normal lung tissue and in emphysematous lung tissue obtained at day 3 or 42 following porcine pancreatic elastase treatment of mice using fluorescent recovery after photobleaching (FRAP), as described in Ref. 32. Each bar represents the mean and SD of 50–100 individual FRAP curve measurements. The effect of static uniaxial strain on K_off is significant (P < 0.001). Additionally, independent of strain, the effects of treatment are also significant (P < 0.001), with an interaction that almost reached the significance level (P = 0.052) (Sato S, Bartolak-Suki E, Suki B, unpublished observations).