Figure 2. Protein expression and thermal characterization.

(A) Polypeptide sequences of (a) [V₁₄]₆ and (b) [AP1-V₁₂]₆, expressed proteins were visualized by Coomassie Blue staining after SDS-PAGE analysis. Left lane: protein molecular weight marker (in kDa). Expected sizes of [V₁₄]₆ (∼35 kDa) and [AP1-V₁₂]₆ (∼37 kDa) are indicated on the right. (B) The turbidity profiles of [V₁₄]₆ and [AP1-V₁₂]₆ protein verses OD₃₅₀ as a function of temperature (increased at a rate of 1°C min⁻¹) were obtained.