Table 1. Dose dependent endothelial cell apoptosis.
Treatment | Concentration | Time [min] | APC positive/PI negative cells [%] |
Non-treated control | 3.15±0.64* | ||
MB | 10 µM | 30 | 5.48±1.91* |
120 | 6.42±2.18* | ||
30 µM | 30 | 9.60±2.99 | |
120 | 19.10±5.00 | ||
60 µM | 30 | 23.80±4.80 | |
120 | 52.05±9.85 * |
The number of apoptotic human microvascular endothelial cells significantly increases in a dose dependent manner (120 min 60 µM vs. control, 30 min 10 µM and 120 min 10 µM) but remains stable regarding the incubation time (* p<0.05, data represent mean±SEM).