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. 2013 Dec 10;8(12):e82308. doi: 10.1371/journal.pone.0082308

Figure 2. Outlet obstruction and transforming growth factor β (TGF-β1) stimulation leads to reduced expression of miR-29.

Figure 2

Time courses of (A) miR-29c and (B) miR-29b expression following rat bladder outlet obstruction. Data are from miRNA microarrays (n=6−8). Independent confirmation of reduced (C) miR-29c and (D) miR-29b in the obstructed bladder by real-time quantitative polymerase chain reaction (n=6). Expression of (E) miR-29c and (F) miR-29b in vehicle-treated (control) and TGF-β1-treated human urinary bladder smooth muscle cells. Cells were treated with 5 ng/ml TGF-β1 in passages 3−5 (six independent replicates using cells from one individual). * p < 0.05 versus control in C through F. ** p < 0.01 versus sham in A. *** p < 0.001 versus sham in B. § p < 0.05 versus 10 d of obstruction in A and B. De-obs = de-obstructed; sham = sham-operated controls.