Table 3. IHC scores of IBD positive tissues fixed in 10% NBF over different time period.
|
Block number
|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Fixation Time (day) |
2 d | 7 d | 8 d | 9 d | 15 d | 23 d | 32 d | 39 d | 50 d | 58 d | |
| Slide 1 | Liver | 2 | 2 | 2,3 | 3 | 2,3 | 3 | 2,3 | 1,2 | 1,2 | N/A |
| Kidney | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2,3 | 2,3* | 3 | |
| Pancreas | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | |
| Slide 2 | Liver | 2 | 3 | 2,3 | 2 | 2,3 | 3 | 3 | 1,2 | 2 | N/A |
| Kidney | 3 | 3 | 3 | 2,3 | 3 | 3 | 3 | 2,3 | 3 | 3 | |
| Pancreas | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | |
| Slide 3 | Liver | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | N/A |
| Kidney | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Pancreas | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
The IHC score was not uniformed in some samples, in which two scores were given.
* In some areas the inclusion bodies were not stained by anti-IBDP MAB. N/A: sample not available.