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. 2013 Dec 10;8(12):e82968. doi: 10.1371/journal.pone.0082968

Figure 2. Klotho reduced ISO-induced apoptosis and ROS production.

Figure 2

(A) Quantitative plot of TUNEL positive cells in CON, ISO and ISO+KL groups for 2, 5, 9 days. Apoptosis rate was determined by the ratio of apoptotic nuclei to total nuclei. The average total cells for each section are about 2000. Data are mean±SEM, n=3. * P<0.05 between two compared groups; NS, no significance. (B) Representative images of Dihydroethidium (DHE) staining of LV sections in CON, ISO and ISO+KL groups for 9 days . Red fluorescence represented the ROS production.200×. (C) Quantitative plot of relative DHE fluorescence intensities normalized by CON. Data are mean±SEM, n=3. * P<0.05 between two compared groups.