Figure 6. In vivo adoptive transfer model isolates lymphocyte outcome of each strain.

Irradiated WT or TLR9^-/- recipient mice were reconstituted by naive WT or TLR9^-/- donor lymphocytes, along with CCl₄ fibrosis induction, as described in Materials and Methods. Accordingly, there were 4 groups of the WT recipients and another 4 groups of TLR9^-/- recipients. Plain and black bars are reflecting naïve recipients reconstituted with WT or TLR9^-/- donor lymphocytes, respectively. Horizontal and vertical gradient bars reflect fibrotic recipients reconstituted with WT or TLR9^-/- donor lymphocytes, respectively. Recipient livers were assessed for histology collagen area, and serum ALT levels were measured. A) Collagen area significantly increased following CCl₄ induction in both groups. Significant fibrosis exacerbation among WT recipients was observed when donor of lymphocytes was TLR9^-/- compared to WT lymphocytes (P<0.01). B) ALT serum levels significantly increased (P<0.01) following fibrosis compared to naive recipients. TLR9^-/- fibrotic recipients reconstituted with TLR9^-/- or C) WT lymphocytes achieved a significant fibrosis (P<0.01) and D) serum ALT (P<0.02) progression when compared to naives. However, both fibrotic groups were similar and showed no hepatic fibrosis or serum ALT changes.