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. 2013 Dec 10;8(12):e82997. doi: 10.1371/journal.pone.0082997

Figure 2. Activation of AT2R/p-ERK/eNOS/NO Pathway in BMMNCs upon AT2R Stimulation.

Figure 2

(A) Dynamic change of ERK phosphorylation after 10 nM CGP42112A treatment at different time points. n=3-4 for each group; *P < 0.05 versus baseline (0 min). (B) Dynamic change of ERK phophorylation after 10 nM CGP42112A and 1μM PD123319 co-treatment at different time points. n=5 for each group. (C) Expression of ERK phosphorylation in preconditioned BMMNCs. n=6 for each group; *P < 0.05 versus BMMNCs group, # P < 0.05 versus BMMNCs+AngII+Valsartan+PD123319 group. (D) eNOS expression of preconditioned BMMNCs was assessed by Western blot. n=6 for each group; *P < 0.05 versus all other groups. (E) NO production in cultured medium was determined with a commercial kit. BMMNCs+CGP42112A group n=3; and n=4 for other groups. *P < 0.05 versus all other groups.