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. 2013 Dec 10;8(12):e82997. doi: 10.1371/journal.pone.0082997

Figure 3. AT2R/p-ERK/eNOS/NO Pathway was Involved in Cardioprotection of BMMNC in vitro.

Figure 3

(A to I) BMMNCs were initially pre-incubated with DMEM, CGP42112A±U0126, CGP42112A±L-NAME, AngII+Valsartan±U0126, AngII+Valsartan±L-NAME, or AngII+Valsartan+PD123319 for 2 hours at 37 °C, respectively. Then preconditioned BMMNCs were co-cultured with NRCMs under hypoxia in serum free medium for 48 hours. Apoptotic NRCMs were detected using TUNEL assay. Bar=100μm; Green represents TUNEL positive cells; Red represents Troponin T (TnT); Blue represents nuclei; and yellow arrowhead represents apoptotic NRCMs. (J) Quantification of apoptotic NRCMs. Single culture NRCMs group n=6; and other groups n=4 for each group; *P < 0.05 versus all other groups.