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. 2013 Dec 10;8(12):e82997. doi: 10.1371/journal.pone.0082997

Figure 6. In vivo.

Figure 6

Effect of AT2R Stimulated BMMNCs on Protection of Adult Cardiac Cells 3 days After Myocardial Infarction.

(A to E) TUNEL assay of adult cardiac cells in peri-infarct region of heart tissue. (Bar=100μm; Green represents TUNEL positive cells; Blue represents nuclei; yellow arrowhead represents apoptotic cells). (F) Quantification of TUNEL positive cells. DMEM group n=6; BMMNCs group n=7; BMMNCs+AngII+Valsartan group n=5; BMMNCs+CGP42112A group n=6; and BMMNCs+AngII+Valsartan+PD123319 group n=4. *P < 0.05 versus DMEM group and BMMNCs group, # P < 0.05 versus BMMNCs+AngII+Valsartan+PD123319 group. (G) Western blot analysis of several apoptotic related proteins expression level (Bax, Bcl2, cleaved caspase3, cleaved caspase9, p-Akt, Akt) in border zone respectively. Rat GAPDH was used as control. Quantification of protein level using densitometry analysis (sham n=3; n=3-5 for other each group). *P < 0.05 versus DMEM group and BMMNCs group; # P < 0.05 versus BMMNCs+AngII+Valsartan+PD123319 group.