Figure 2. The upper interface pivotal T248 residue affects channel maturation and gating.

A) K⁺ currents recorded from Xenopus laevis oocytes expressing the wild type channel, the T248A and L249T single-mutants and the corresponding T248A;L249T double-mutant. B) Voltage-activation data for the K⁺ channel proteins indicated in (A). In all panels, smooth curves correspond to a two-state Boltzmann function. For clarity, in this and in other figures, no error bars are plotted for the different data points of the different curves. These values, however, are in a range of 5% of the reported values.