Figure 6. Upper interface mutations do not affect slow inactivation gating.

A) A typical command protocol, along with the potassium currents elicited from the wild type and upper interface T248A, S428A and Y415A mutants during the onset of slow inactivation (normalized relative to the peak current amplitude). B) A typical command protocol, along with the elicited potassium currents for the wild type and upper interface T248A, S428A and Y415A mutants during the onset of and recovery from slow inactivation, is depicted. C) Normalized kinetics for the recovery from inactivation of the different mutants indicated in (B). The data were fitted to a double-exponential function. The time constants of the slow phase (indicative of recovery from inactivation (see Materials and Methods)) for the T248A, Y415A and S428A mutants are reported in Table 4 .