. 2013 Dec 10;8(12):e82253. doi: 10.1371/journal.pone.0082253

Table 1. The influence of upper interface T248 and L249 mutations on activation gating of the Shaker Kv channel^a .

Channel protein^b	K⁺ current?	V_1/2 (mV)	Z	ΔG _open (kcal/mol)
Wild type	Observed	−21.6±0.2	3.20±0.07	−1.64±0.04
T248A	NO	NA	NA	NA
L249T	Observed	−19.2±0.2	3.04±0.05	−1.36±0.02
T248A;L249T	Observed	6.4±0.2	1.89±0.02	0.28±0.01

Fig. 2 .^a The table displays the gating parameters of all single- and double-mutants used to assess the effect of T248 and or L249 mutation on channel maturation and gating, as addressed in

^b Mutants were generated using a monomeric gene construct such that all four subunits carry the indicated mutation.

Table 1. The influence of upper interface T248 and L249 mutations on activation gating of the Shaker Kv channela .

Table 1. The influence of upper interface T248 and L249 mutations on activation gating of the Shaker Kv channel^a .