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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: J Neurosci Methods. 2013 Sep 19;221:10.1016/j.jneumeth.2013.09.005. doi: 10.1016/j.jneumeth.2013.09.005

Figure 1.

Figure 1

Generation of hDβH-EGFP transgenic mice. A, Schematic diagram of the hDβH-EGFP transgene. mPrm-1 denotes murine protamine-1. B, Portion of a midsection through a stellate ganglion from an adult hDβH-EGFP transgenic mouse (line 4) stained for expression of tyrosine hydroxylase (TH; red) and EGFP (green), and counterstained with nuclear Hoechst (blue). The three-channel merged image is shown in the right panel. TH-expressing cells exhibit both cytoplasmic and nuclear anti-EGFP immunofluorescence. C, Section through a control stellate ganglion stained as in the transgenic ganglion, showing TH-expressing and non-expressing cells. D, Volume renderings of a 30-slice confocal z-stack obtained from a 10-μm-thick section from the left ventricular free wall of an adult hDβH-EGFP heart (line 4) dually stained with an antiTH and an anti-GFP antibody, followed by exposure to Alexa546- (red) and Alexa633- (green) conjugated secondary antibodies. The yellow color in the merged image indicates co-localization of TH and EGFP. The right panel is a magnified view of the boxed region in the merged image.