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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Carcinog. 2013 Aug 31;53(0 1):E130–E139. doi: 10.1002/mc.22054

Figure 6.

Figure 6

Overexpression of Slug inhibits calcitriol’s radiation sensitizing ability. (A) Real-time RT-PCR confirmed expression of Snail and Slug in stable transfected pools of both Snail and Slug overexpressing DLD-1 (black bars) and HCT116 (gray bars) cells compared to the empty vector (EV) controls. Data were normalized to GAPDH. (B) Western Blot analysis of VDR and E-cadherin from total cell lysates of Snail and Slug expressing HCT116 and DLD-1 cells compared to the empty vector controls. GAPDH was used as a loading control. (C) Cell lines were pre-treated with 1 μM calcitriol (gray bars) or vehicle alone (black bars) for 24 h prior to radiation treatment of 0–6 Gy and compared with empty vector stably transfected cells. After 10 d, surviving colonies of 20 or more cells were counted to determine the % survival relative to the untreated cells. *P < 0.01, **P < 0.05.