Fig. 4.

Spontaneous astrocytic Ca²⁺ activity in awake mice is dependent upon activation of the α₁-AR. (A) Representative astrocytic rhod-2 fluorescence traces show frequent widespread and coordinated astrocytic (Ca2+)i waves in awake animals, which were almost completely abrogated by the α₁-AR antagonist, prazosin, and LC toxin, DSP4. (B) Bar graph showing that prazosin and DSP4 treatment causes a significant decrease in the frequency of coordinated astrocytic [Ca²⁺]_i waves (quantified by counting each time >1/3 of cells responded within the imaging field) ** p<0.01, * p<0.05, paired t-test (Control versus prazosin), unpaired t-test (control versus DSP4); [n=4 animals (prazosin, control), 5 animals (DSP4)] Data are shown as mean ± SEM.