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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 6;191(12):10.4049/jimmunol.1302273. doi: 10.4049/jimmunol.1302273

Figure 1. Reciprocal development of diabetes and colitis in NOD.scid recipients is dependent on the age of NOD donor thymus.

Figure 1

(A) Cytokeratin-8 (green, cortex) and UEA-1 (red, medulla) immunostaining of sections from newborn, 7 d-, 10 d-, and 4 wk-old NOD thymi; images were taken at 100X magnification. (B) NOD.scid mice were engrafted with thymi from newborn (NB) or 12 wk-old (n=10) NOD female donors and the frequency of CD4+ (left panel) and CD8+ (right panel; *p<10-4) T cells measured in peripheral blood. (C) Diabetes (left panel; *p<10-4 versus recipients of 7 d or older thymi; Kaplan-Meier Log Rank) and the frequency of insulitis (right panel; n=8; *p<10-4; Student’s t test) in thymus recipients; representative H&E staining of pancreatic sections of recipients of newborn (D) and 12 wk-old thymi (E). (F) Body weight (left panel; *p<10-4; 2-way ANOVA) and colitis scores (right panel; *p<10-4; Student’s t test) of recipients (n=8) of different aged thymi and control NOD.scid littermates. Representative Alcian blue stained colonic sections of recipients of newborn (G) and 12 wk-old (H) thymi. Representative H&E staining of thyroid (I,J) and salivary (K,L) glands from newborn (I,K) and 12 wk-old (J,L) thymus recipients. Error bars represent SEM.