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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 6;191(12):10.4049/jimmunol.1302273. doi: 10.4049/jimmunol.1302273

Figure 3. Colitogenic T cells respond to intestinal microbial antigens.

Figure 3

(A) Secretion of IFNγ and IL-17 by isolated T cells stimulated with CBL, as measured by ELISA, in the spleen, PLN, and MLN of recipients of different aged thymi 8 wk post-implantation (n=7; *p<10-4; Student’s t test). (B) Splenic CD4+ and CD8+ T cells isolated from NOD.scid thymus recipients 8 wk post-implantation, and adoptively transferred into NOD.scid mice, which were monitored for body weight loss (left panel; *p<0.05, 2-way ANOVA) and development of rectal prolapse (right panel; *p<0.05, 2-way ANOVA). (C) Representative colonic sections stained with Alcian blue, and colitis scores (*p<0.05; Student’s t test) and (D) diabetes incidence for recipients (n=5) of thymi from 6 wk-old NOD.BDC2.5 and NOD.BDC2.5Cαnull donors. Error bars represent SEM.