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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 13;191(12):10.4049/jimmunol.1301329. doi: 10.4049/jimmunol.1301329

Figure 6. Assessment of activation of classical and alternative NF-κB pathways in human lung epithelial cells exposed to HDM.

Figure 6

(A) Primary human nasal epithelial (PHNE) cells or (B) Human bronchial epithelial (HBE) cells were exposed to PBS or 25 μg HDM once a day for consecutive days and harvested thereafter at either 72 h (PHNE) or 48 h (HBE). Cells were lysed for evaluation of NIK, p52, phospho RelA 536, by Western blot analyses. β-Actin (loading control). Right panels: Densitometric evaluation of Western blots shown in (A) PHNE (n=3 patients) or (B) HBE (n=3 experimental repeats). Results are expressed as arbitrary density and were normalized to corresponding β-Actin bands.