Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 13;191(12):10.4049/jimmunol.1301203. doi: 10.4049/jimmunol.1301203

Figure 2. CpG-induced IL-6 production is downregulated in Bank1−/− B cells, but Tlr9 gene expression is not affected.

Figure 2

(A) IL-6 and IL-10 were measured by ELISA of WT (+/+) and Bank1 KO (−/−) B-cell supernatants collected at the indicated times following CpG, anti-IgM + CpG or CpG + anti-mouse CD40 (10μg/ml). Data shown are mean ± SD of 3 replicates, and is representative of 4 independent experiments. (B) Splenic B cells from Bank1+/+ and Bank1−/− mice were stimulated with 2μM CpG for the indicated times. Relative expression of the Tlr9 gene was analyzed by Taqman real-time PCR. Data shown is the mean ± SEM of 3 replicates from a representative experiment out of 3 performed. (C) Bank1+/+ and Bank1−/− splenic B cells were stimulated with LPS (20 μg/ml) or R848 (1 μg/mL), or left unstimulated for the times shown. Data are the representative of two independent experiments with 3 replicates each. Bars represent mean ± SD.