Figure 6. C4 reduced tumor growth in vivo in mouse orthotopic model of pancreatic cancer.

A. Orthotopic MiaPaCa-2 xenografts established in SCID mice. 1x10⁶ MiaPaCa-2 cells, stably transfected with Luciferase expressing plasmid, were injected into the pancreas of SCID mice (n=6). Bioluminescence (BL) imaging was performed at days 7, 14 and 21 after tumor cell injection. B. Tumor burden (measured by BLI; photon flux) at the end of the experiment. C. Western blot of tumor lysates probed with P-FAK (Y397), P-Akt (S473), P-Erk1/2 (T202/Y204) and P-Paxillin (Y118) antibody. D. Orthotopic model with Human Pancreatic Tumor 11424. Mice treatment with C4 started on day 42 post implantation and followed for 21 days. MRI was performed once a week after day 20 post implantation. Bar graph shows fold change in tumor volume (measured by MRI) in PBS and C4-treated animals at 3 weeks post treatment compared to baseline pretreatment estimates.