Figure 5. Effects of CCCI-01 in colony formation of cancer and normal cells.

(A) Clonogenic assay was carried out in BT-549 and MCF-10A treated with CCCI-01. Colonies consisting of more than 50 cells were counted after 9 days of incubation with CCCI-01 or DMSO. All treatments received 0.06 % DMSO. Surviving fraction was obtained by normalizing to DMSO control (= 1). Values are average from at least two independent experiments. P, student's t-test. The blue line in the graph indicates IC₅₀. (B and C) The CFC assay was carried out in primary normal bone marrow cells. Cells were cultured in the presence of varying concentrations of CCCI-01. (B) Surviving fractions of CFC-G/M and BFU-E/CFU-E colonies were obtained by normalizing to the DMSO control (= 1). Values are average of two independent experiments with two normal bone marrow samples: one is nomonuclear cells and the other is selected for CD34+. Separate data are available in Supplemental Table 1. For CFC-G/M, no inhibitory effects were detected up to 1 μM, and colony size and number were reduced in both samples at 10 μM. BFU-E/CFU-E colonies did not seem to be affected up to 3 μM, and showed inhibition in colony formation at 10 μM in both samples. The blue line in the graph indicates IC₅₀. (C) Representative images of each type of colonies treated with different concentrations of CCCI-01.