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. 2013 Sep 25;4(10):1763–1776. doi: 10.18632/oncotarget.1198

Figure 6. Effects of CCCI-01 on spindle polarity in BT-549 cancer cells and normal primary HMECs.

Figure 6

Centrosome arrangement and spindle multipolarity were examined by immunofluorescence after treating cells with CCCI-01 for 5 hours. As little as 5 μM increased centrosome de-clustering to 70 % in BT-549 breast cancer cells (average of two independent experiments, at least 30 mitotic cells/treatment/experiment). In contrast, even at 8 μM, de-clustering was not induced (P > 0.7, student's t-test) in normal primary HMEC from three different reduction mammoplasty samples (average of two independent experiments with three HMEC samples in total, at least 30 mitotic cells/treatment/sample). Scale bar = 10 μm. *P < 0.02, student's t-test.