Figure 7. Synthetic P9 peptides inhibit bFGF-induced MAP kinase activation.
Panel A. Before stimulation with 30 ng/ml bFGF for 20 min, starved B16-F10 cells were pretreated with P9 at the indicated concentrations for 5 min. Cell lysates were resolved with 10% SDS-PAGE, transferred to PVDF membrane, and blotted with anti-pErk1/pErk2 mAb. Data shown are the representative pictures of 3 independent experiments. Panel B. Density ratios of pErk1/pErk2 to Erk1/Erk2. Data are presented as the mean (±SDs) of 3 independent experiments.
