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. 2013 Nov 21;9(10):1089–1098. doi: 10.7150/ijbs.7367

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Effects of Genistein on BMP2/SMAD5/RUNX2 transcription and downstream gene expression in cultured hBMSCs. Real-time RT-PCR analyses revealed that five representative genes (A) BMP2, (B) SMAD5, (C), RUNX2, (D), ALP, and (E) OSTEOCALCIN (OSC) were differentially expressed in Genistein (GEN, 10^-6M)-treated and vehicle control hBMSC cultures in the absence or presence of a complete estrogen receptor (ER) antagonist, ICI182780 (ICI, 10^-7M). Consistent with the results of microarray analysis, normalized transcript levels of BMP2, SMAD5, RUNX2, ALP, and OSC are shown to be upregulated in GEN-treated cells, and these five genes have significant difference between GEN-treated and the vehicle control group. In addition, ER antagonist ICI completely abolished the GEN-induced osteogenic gene transcription in cultured hBMSCs. Data are mean ± S.D. from triple independent experiments. Values sharing the same superscript are not significantly different at P<0.05.