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. 2013 Nov 21;9(10):1089–1098. doi: 10.7150/ijbs.7367

Figure 4.

Figure 4

Effects of gene-specific siRNAs knock-down on Genistein-induced BMP2/SMAD5/RUNX2 signaling in cultured hBMSCs. (A) Representative western blot for BMP2, SMAD5, RUNX2, ALP, and OSTEOCALCIN (OSC) protein expressions in Genistein-treated and untreated hBMSC cultures after transfected with different gene-specific siRNAs. (B) Band densitometry quantified by fluorChem 8900 and normalized to β-ACTIN. Consistent with microarray analysis and real-time RT-PCR results, siRNAs directed against BMP2, or SMAD5, or RUNX2 significantly attenuated Genistein-induced increments of BMP2, SMAD5, RUNX2, ALP, and OSC protein expression, respectively, when compared with control siRNA treatment. Data are mean ± S.D. from triple independent experiments. Values sharing the same superscript are not significantly different at P<0.05.