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. 2013 Nov 28;9(10):1108–1120. doi: 10.7150/ijbs.7232

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Identification of NSPCs (neurospheres) derived from mouse brain. The left image of the upper panel shows the cultured NSPCs grow in a manner of neurospheres. The middle and the right of the upper panel show that cells of neurospheres are Nestin-positive and Nestin/Hoechst double staining, respectively. The lower panel shows the Tuj 1- (for neuron, 4 days after differentiation), GFAP- (for astrocyte, 4 days after differentiation), and CNPase- (for oligodendrocyte, 10 days after differentiation) positive cells differentiated from neurospheres, respectively.