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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Biochemistry. 2013 Nov 4;52(46):10.1021/bi401116n. doi: 10.1021/bi401116n

Figure 4. Effect of ACC activity on the initial reaction velocity of the complete reconstituted system shown in Figure 1.

Figure 4

(A) Titration of AccAD complex. (B) Titration of AccB subunit. (C) Titration of AccC subunit. Reactions contain varying concentrations of ACC subunits or complex in question, 1 μM FabABDFGH, 10 μM FabIZ, 30 μM holo-ACP, 30 μM TesA (TE), 600 μM acetyl-CoA, 15 mM sodium bicarbonate, 1 mM ATP, 5 mM MgCl2, and 1.3 mM NADPH.