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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Biochemistry. 2013 Nov 4;52(46):10.1021/bi401116n. doi: 10.1021/bi401116n

Figure 5. Kinetic properties of overall reconstituted pathway shown in Figure 1.

Figure 5

(A) Kinetic linearity of overall reconstituted pathway. Reaction conditions: 2–10 μM AccAD, AccC, 6–30 μM holo-AccB, 0.2–1 μM FabABDFGH, 2–10 μM FabIZ, 6–30 μM holo-ACP, 6–30 μM TesA, 600 μM acetyl-CoA, 15 mM bicarbonate, 1 mM ATP, 5 mM MgCl2, and 1.3 mM NADPH. (B) Palmitic acid product formation in 5-fold dilution system. (C) Initial reaction rates of optimized reconstituted systems with an individual enzyme concentration doubled. Experiments were performed in two-fold dilution system.