Figure 8. GC-FID chromatographic analysis of product profiles under varying FabB:FabA ratios.
The relevant assay conditions are: (1) 1 μM FabB, 10 μM FabA; (2) 1 μM FabB, 1 μM FabA; (3) 3 μM FabB, 1 μM FabA; (4) 5 μM FabB, 1 μM FabA; (5) 7 μM FabB, 1 μM FabA; (6) 10 μM FabB, 1 μM FabA; and (7) 10 μM FabB, 10 μM FabA. Chromatographic peaks were identified through comparison of retention time with authentic standards.